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CRL-2266 SH-SY5Y 人神經(jīng)母細胞瘤細胞，原代細胞|細胞系|細胞株|菌種；細胞庫管理規(guī)范，提供的細胞株背景清楚，提供參考文獻和培養(yǎng)條件！
CRL-2266 SH-SY5Y ，人神經(jīng)母細胞瘤細胞，細胞
CRL-2266 SH-SY5Y ，人神經(jīng)母細胞瘤細胞，細胞
CRL-2266 SH-SY5Y ，人神經(jīng)母細胞瘤細胞，細胞
CRL-2266 SH-SY5Y ，人神經(jīng)母細胞瘤細胞，細胞

CRL-2266 SH-SY5Y 人神經(jīng)母細胞瘤細胞

ATCC® Number:	CRL-2266?	Price:
Designations: SH-SY5Y Depositors: JL Biedler Biosafety Level: 1 Shipped: frozen Medium & Serum: See Propagation Growth Properties: mixed, adherent and suspension Organism: Homo sapiens (human) Morphology: epithelial Source: Organ: brain Disease: neuroblastoma Derived from metastatic site: bone marrow Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. Restrictions: NOTE: SH-SY5Y was deposited at the ATCC by June L. Biedler, Memorial Sloan-Kettering Cancer Center. SH-SY5Y is distributed for academic research purposes only. Memorial Sloan-Kettering releases the line subject to the following: 1.) SH-SY5Y or its products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of SH-SY5Y including any use by a for-profit entity must first be negotiated with Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439. Isolation: Isolation date: 1970 Applications: transfection host (Roche FuGENE® Transfection Reagents technology from amaxa) Antigenexpression: Blood Type A; Rh+ DNA Profile (STR): Amelogenin: X CSF1PO: 11 D13S317: 11 D16S539: 8,13 D5S818: 12 D7S820: 7,10 THO1: 7,10 TPOX: 8,11 vWA: 14,18 Cytogenetic Analysis: modal number = 47; the cells possess a unique marker comprised of a chromosome 1 with a complex insertion of an additional copy of a 1q segment into the long arm, resulting in trisomy of 1q [22554] Age: 4 years Gender: female Comments: SH-SY5Y cells have a reported saturation density greater than 1 X 10(6) cells/sq cm. They are reported to exhibit moderate levels of dopamine beta hydroxylase activity [PubMed ID: 29704]. Propagation: ATCC complete growth medium: The base medium for this cell line is a 1:1 mixture of ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C Subculturing: Protocol: These cells grow as a mixture of floating and adherent cells. The cells grow as clusters of neuroblastic cells with multiple, short, fine cell processes (neurites). Cells will aggregate, form clumps and float. Remove the medium with the floating cells, and recover the cells by centrifugation. Rinse the adherent cells with fresh 0.25% trypsin, 0.53 mM EDTA solution, add an additional 1 to 2 ml of trypsin solution, and let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate, combine with the floating cells recovered above and dispense into new flasks. Subcultivation Ratio: A subcultivation ratio of 1:20 to 1:50 is recommended Medium Renewal: Every 4 to 7 days Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase Doubling Time: 48 hrs Related Products: recommended serum:ATCC 30-2020 parental cell line:ATCC HTB-11 References: 22554: Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. PubMed: 6137586 23032: Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. PubMed: 29704

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iec-6 大鼠小腸隱窩上皮細胞
馬-達二氏犬腎細胞系 MDCK (NBL-2) CRL-34
鴨子胚胎成纖維細胞  Duck embryo  CCL-141
小鼠胚胎成纖維細胞 3T3-L1 CCL-173
人結(jié)腸直腸腺癌 COLO 320DM CCL-220
人結(jié)腸直腸腺癌 NCI-H508 CRL-253
人結(jié)腸直腸腺癌 COLO 320HSR CCL-220.1
人B淋巴細胞 Ramos（RA1） CRL-1596
人淋巴母細胞 T2(174×CEM.T2) CRL-1992
大鼠心肌細胞 H9C2(2-1) CRL-1446
CRL-2266 SH-SY5Y 人神經(jīng)母細胞瘤細胞
豬鼻甲黏膜成纖維細胞 PT-K75 CRL-2528
小鼠胰腺癌細胞 LTPA CRL-2389
大鼠胰腺癌細胞 DSL-6A/C1 CRL-2132
小鼠骨髓瘤B淋巴細胞 SP2/MIL-6 CRL-2016
大鼠腦間質(zhì)細胞 DI TNC1 CRL-2005
小鼠肌原細胞 C2C12 CRL-1772
鵪鶉纖維肉瘤細胞 QT6 CRL-1708
大鼠回腸細胞 IEC-18 CRL-1589
非洲綠猴腎細胞 VERO C1008 [Vero 76, clone E6, Vero E6] CRL-1586
人成纖維細胞 Hs 738.St/Int CRL-7869
人正常前列腺上皮細胞 WPE-int CRL-2888
人正常前列腺成肌纖維細胞 WPMY-1 CRL-2854
人胰腺導(dǎo)管腺癌細胞 PL45 CRL-2558
人胰腺癌細胞 Panc 05.04 CRL-2557
人胰腺癌細胞 Panc 04.03 CRL-2555
人胰腺癌細胞 Panc 02.03 CRL-2553
人胰腺癌細胞 Panc 08.13 CRL-2551
人胰腺癌細胞 Panc 03.27 CRL-2549
人胰腺癌細胞 Panc 10.05 CRL-2547
人肝癌細胞 SNU-398 CRL-2233
人成神經(jīng)瘤細胞-骨髓 SK-N-DZ CRL-2149
人胰腺癌細胞 HPAC CRL-2119
人胰腺癌細胞 HPAF-II CRL-1997
人黑色素瘤細胞 A375.S2 CRL-1872
人臍靜脈內(nèi)皮細胞 HUV-EC-C CRL-1730
人黑素瘤細胞 WM-266-4 CRL-1676
人膀胱癌細胞 HT-1376 CRL-1472
人正常前列腺上皮細胞 RWPE-2 CRL-11610
人正常前列腺上皮細胞 RWPE-1 CRL-11609
雞胚胎成纖維細胞 UMNSAH/DF-1 CRL-12203
人乳腺癌細胞 MDA-MB-231 HTB-26
小鼠巨噬細胞 RAW 264.7 TIB-71
人橫紋肌肉瘤細胞 A-204 HTB-82
人胰腺癌細胞 Capan-2 HTB-80
人胰腺癌細胞 Capan-1 HTB-79
人乳腺導(dǎo)管癌細胞 MDA-MB-435S HTB-129
人胃癌細胞 Hs 746T HTB-135
人肺腺癌細胞 NCI-H441 [H441] HTB-174
人骨髓瘤細胞 U266 TIB-196
人結(jié)腸癌細胞 NCI-H716 [H716] CCL-251
人多形性惡性膠質(zhì)瘤 T98G [T98-G] CRL-1690
人神經(jīng)母細胞瘤細胞 BE(2)-C CRL-2268
人腎透明細胞癌細胞 Caki-2 HTB-47
人皮膚成纖維細胞 Hs 865.Sk CRL-7601
CA46 人淋巴瘤細胞系
CRL-5826 NCI-H226 人肺鱗癌細胞系
Tca-8113 人舌鱗癌細胞
MA-891 小鼠乳腺癌高轉(zhuǎn)移細胞
U14 小鼠宮頸癌細胞
CRL-9518 L5178Y 小鼠淋巴瘤細胞
HTB-112 HEC-1-A 人子宮內(nèi)膜腺癌細胞
HTB-113 HEC-1-B 人子宮內(nèi)膜腺癌細胞
AN3 CA 人子宮內(nèi)膜腺癌細胞
ROS1728 大鼠成骨細胞
CRL-6323 B16F1 小鼠黑色素瘤細胞
HTB-58 SK-MES-1 人肺鱗癌細胞
CCL-127 IMR-32 人神經(jīng)母細胞瘤細胞
HTB-79 Capan-2 人胰腺癌細胞
BALL-1 人B淋巴細胞急性白血病細胞
CRL-2922 Eayh926 人臍靜脈細胞融合細胞
WB-F344 大鼠肝干細胞
CRL-1715 TM4 正常小鼠睪丸細胞
CRL-2539 4T1 小鼠乳腺癌細胞
CRL-1442 BRL-3A 大鼠肝細胞
BRL 大鼠肝細胞
RSC96大鼠雪旺細胞
Isk 人子宮內(nèi)膜癌細胞
HTB-113 hec-1-b 人子宮內(nèi)膜腺癌細胞
PLA-802?人惡性橫紋肌肉瘤
Pt-K2 袋鼠腎細胞
BS-C-1 非洲綠猴腎細胞
A498人腎癌細胞
3AO人卵巢癌細胞
iec-6 大鼠小腸隱窩上皮細胞
u87MG 人腦星形膠質(zhì)母細胞瘤
Renca 小鼠腎癌細胞
nci-h929 骨髓瘤細胞
P388小鼠白血病細胞
opm-2 骨髓瘤細胞
MDA-MB-435 人乳腺癌高轉(zhuǎn)移細胞
KU812 人外周血嗜堿性白細胞
NIH:OVCAR-3 人卵巢癌細胞
SW 1990人胰腺癌細胞系
S180人結(jié)腸腺癌細胞
HuTu-80十二指腸腺癌
RL95-2 人子宮內(nèi)腺癌細胞
NCI-H446 人小細胞肺癌細胞
MSTO-211H 人肺癌細胞
H4-Ⅱe 大鼠肝癌細胞系
HLF 人肺成纖維樣細胞
LS 180人結(jié)腸腺癌細胞
HuTu-80十一指腸腺癌
ST豬睪丸細胞系
REH人急性非B非T淋巴細胞白血病
NCI-H520 人肺鱗癌細胞
RKO 人結(jié)腸腺癌細胞
293A 人胚腎細胞
RK-13 兔腎細胞系