中文名稱  DNA甲基轉(zhuǎn)移酶3L抗體
別    名  Cytosine 5 methyltransferase 3 like protein; DNA (cytosine 5 ) methyltransferase 3 like; DNA (cytosine-5)-methyltransferase 3-like; DNA Cytosine 5 Methyltransferase 3 Like Protein; DNA Methyltransferase 3 Like Protein; DNM3L_HUMAN; Dnmt 3L; Dnmt3l; Human cytosine 5 methyltransferase 3 like protein; MGC1090.   
濃    度  1mg/1ml
規(guī) 格  0.2ml/200μg          
抗體來(lái)源  Rabbit  
克隆類型  polyclonal
交叉反應(yīng)  Human, Mouse, Rat   
產(chǎn)品類型  一抗    
研究領(lǐng)域  細(xì)胞生物 表觀遺傳學(xué)  
蛋白分子量  predicted molecular weight: 44kDa
性    狀  Lyophilized or Liquid
免 疫 原  KLH conjugated synthetic peptide derived from human Dnmt3L
亞    型  IgG
純化方法  affinity purified by Protein A
儲(chǔ) 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
產(chǎn)品應(yīng)用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500
（石蠟切片需做抗原修復(fù)）
 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user.  
保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

DNA甲基轉(zhuǎn)移酶3L抗體產(chǎn)品介紹
 Methylation at the 5'-position of cytosine is the only known naturally occurring covalent modification of the mammalian genome. DNA methylation requires the enzymatic activity of DNA 5-cytosine methyltransferase (Dnmt) proteins, which catalyze the transfer of a methyl group from S-adenosyl methionine to the 5'-position of cytosines, thereby repressing expression of the target gene. Dnmt3L (DNA (cytosine-5)-methyltransferase 3-like) is a 387 amino acid protein that contains one ADD-type zinc finger and is a member of the Dnmt family. Localized to the nucleus and expressed at lows levels in thymus, testis and ovary, Dnmt3L does not exhibit DNA methyltransferase activity, but is able to stimulate de novo methylation by Dnmt3 and is thought to play a key role in the establishment of genomic imprints. Additionally, Dnmt3L interacts with histone deacetylase 1 (HDAC1) and, through this interaction, mediates transcriptional repression. Multiple isoforms of Dnmt3L exist due to alternative splicing events.

Function : Catalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A and DNMT3B. Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4) and induces de novo DNA methylation by recruitment or activation of DNMT3.

Subunit : Homodimer. Heterotetramer composed of 1 DNMT3A homodimer and 2 DNMT3L subunits (DNMT3L-DNMT3A-DNMT3A-DNMT3L). Interacts with histone H3 (via N-terminus).

Subcellular Location : Nucleus.
Tissue Specificity : Expressed at low levels in several tissues including testis, ovary, and thymus.
Similarity : Belongs to the C5-methyltransferase family.
Contains 1 ADD-type zinc finger.
Database links : UniProtKB/Swiss-Prot: Q9UJW3.3

【存儲(chǔ)要求】連續(xù)使用時(shí)4°C存儲(chǔ)，保質(zhì)期六個(gè)月；DNA甲基轉(zhuǎn)移酶3L抗體期存儲(chǔ)時(shí)建議分裝為10ul以上小包裝-20°C存儲(chǔ)，并避免反復(fù)凍融，保質(zhì)期一年。

實(shí)驗(yàn)的用途：
1）WB：Western Blotting 免疫印跡
2）IH：Immunohistochemistry 免疫組化
3）IH(P)：Immunohistochemistry Parraffin sections 免疫組化(石蠟)
4）IH(F)：Immunohistochemistry Frozen sections 免疫組化(冰凍)
5）IC：Immunocytochemistry 免疫細(xì)胞化學(xué)